La Jolla, CA
| Category: | Research & Development | Type: | New | Division: | Research & Development |
| Relocation: | No | Band Level: | Band 2 – Senior Administrative Professional | ||
Job Description: |
| The International AIDS Vaccine Initiative (IAVI) is a nonprofit scientific and charitable organization founded in 1996 whose mission is to ensure the development of safe, effective, accessible, preventive HIV vaccines for use throughout the world. IAVI's work focuses on four areas: accelerating scientific progress; mobilizing public support through issue advocacy and education; encouraging industrial involvement in AIDS vaccine development; and working to assure global access to a vaccine. IAVI is a young, dynamic and mission-driven organization, and the work environment is fast-paced and intellectually stimulating, relying on the good cheer and high level of cooperation of its committed members. Job Opportunity Job Title: Laboratory Technician Location: NAC Center at TSRI, La Jolla, California The candidate will participate in the execution and interpretation of experiments related to projects focused on the characterization of neutralizing antibodies and antibody responses to HIV-1 infection, and on the mechanisms of neutralization and antibody-mediated protection. Minimum qualifications include M.S. or B.S. degree in cell biology or related field with a minimum 1 year of relevant experience. Any appropriate combination of education, certifications, and/or relevant work experience will be considered. Main responsabilities: - Prepare HIV-1 and pseudotyped virus stocks in BSL3 conditions - Perform viral neutralization assays - Perform viral entry assays - Perform site-directed mutagenesis - Perform cell-binding assays - Perform ELISAs - Maintain cells lines in culture for transfections, virus production, neutralization assays - Prepare and activate human primary cells - Organize effectively and discuss data Required skills. Experience in common techniques in - cellular biology and immunology: cell culture, cell purification, ELISAs … - biochemistry: protein purification, immunoprecipitations, SDS-Page, Western Blots… - molecular biology: RNA, DNA purification, PCR, real time PCR, sequencing, site directed mutagenesis… Physical Demands: The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. Specific vision abilities require by this job include close vision, distance vision, color vision, peripheral vision, depth perception and abilities to adjust focus. Work Environment: The work environment characteristics described here are representative of those an employee encounters while performing the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. IAVI is an equal opportunity employer and encourages applications from diversity candidates. |
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