La Jolla, CA
| Category: | Research & Development | Type: | New | Division: | Research & Development |
| Relocation: | No | Band Level: | Band 2 Senior Administrative Professional | ||
Job Description: |
| The International AIDS Vaccine Initiative (IAVI) is a nonprofit scientific and charitable organization founded in 1996 whose mission is to ensure the development of safe, effective, accessible, preventive HIV vaccines for use throughout the world. IAVI's work focuses on four areas: accelerating scientific progress; mobilizing public support through issue advocacy and education; encouraging industrial involvement in AIDS vaccine development; and working to assure global access to a vaccine. IAVI is a young, dynamic and mission-driven organization, and the work environment is fast-paced and intellectually stimulating, relying on the good cheer and high level of cooperation of its committed members. Job Opportunity Job Title: Laboratory Technician Location: NAC Center at TSRI, La Jolla, California Position Summary: The candidate will perform state-of-the-art molecular biology and recombinant protein expression, production, purification and characterization of HIV-1 envelope glycoproteins (Env) or related proteins. The HIV-1 Env will be used both as immunogens and as reagents for analyzing anti-Env immune responses elicited in relevant small animal models, non-human primates and humans. The candidate may also study the mechanisms of neutralization by different monoclonal antibodies and may work with HIV-1 pseudoviruses under BSL3 laboratory safety conditions. The candidate will be able to design, execute experiments and interpret the data in an independent manner or with limited supervision. Minimum qualifications include a M.S. or B.S. degree in molecular biology or virology or related areas of biological sciences. The candidate will preferably possess 3 years of relevant laboratory research experience, but other highly qualified candidates will be considered. Key Responsibilities: Standard mutagenesis and cloning to generate protein expression vectors Expression and purification of HIV-1 Env and other proteins on a routine basis Perform FACS-based binding assays Perform ELISAs and immunoprecipitation assays Perform viral neutralization assays Maintain cells lines in culture for transfection, virus production and as neutralization targets Prepare pseudotyped virus stocks under BSL3 conditions Isolate PBMCs from selected blood samples Organize effectively and discuss data Order reagents and supplies and display good laboratory practices Experience and Skills: Molecular biology: RNA/DNA purification, PCR, site-directed mutagenesis Biochemistry: protein purification, FPLC, immunoprecipitation, SDS-Page, Western Blots Cellular biology and immunology: cell culture, cell purification, ELISAs, FACS Experience with gel filtration chromatography is a plus Physical Demands: The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. Specific vision abilities require by this job include close vision, distance vision, color vision, peripheral vision, depth perception and abilities to adjust focus. Work Environment: The work environment characteristics described here are representative of those an employee encounters while performing the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. IAVI is an equal opportunity employer and encourages applications from diversity candidates. |
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